Additional Cell Culture Reagents and Supplements
Sigma Aldrich Fine Chemicals Biosciences IL 4 human Animal componen
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation survival and gene expression. Produced by mast cells T cells and bone marrow stromal cells IL-4 regulates the differentiation of naive CD4 T cells into helper Th2 cells characterized by their cytokine-secretion profile that includes secretion of IL-4 IL-5 IL-6 IL-10 and IL-13 which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.
BioVendor, LLC Sircol Acid-Salt Wash Reagent (3 x 20 ml), concentrated, Biocolor Item # SWASH
Sircol; Acid-Salt Wash Reagent (3 x 20 ml), concentrated. Contains acetic acid, sodium chloride and surfactants. Dilute the contents of the vial to 100 ml with deionised water before use. Sircol™ Collagen Assay component. Biocolor Item # SWASH
Research Products International Corp Murashige & Skoog Vitamin Mixture, 10.31 Grams of Powder, Makes 100 Milliliters of Solution
Powdered vitamin mixture as described by Murashige & Skoog (1962). Makes 1000X stock solution. Package contains 103.1 grams of powder per liter of medium. Use at a concentration of 1 ml per liter to achieve final concentration. Hygroscopic.
Sigma Aldrich Fine Chemicals Biosciences TrypZean bovine recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP) | 9002-07-7 | MFCD04041188 | 10mg
TrypZean bovine recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP) | Mol Wt: | 9002-07-7 | MFCD04041188 | 10mg
STEMCELL Technologies Human Platelet Lysate Fibrino
Growth factor-rich supplement for the expansion of cells in vitro
Advanced Biomatrix Vitronectin, Human, 0.1 mg
Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.Vitronectins primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice.
Advanced Biomatrix HyStem-C Version 2.0 Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit, 12.5 mL
HyStem-C contains thiolated hyaluronic acid & gelatin for easy 3D cell culture. HyStem-C version 2.0 is reconstituted with a buffered 1X PBS. The resulting components will also result in a pH neutral isotonic salt hydrogel. The ending pH, osmo, and rheological properties are the same from Version 1.0 to Version 2.0. HyStem-C hydrogels provide an excellent starting point for optimizing the matrix for cell culture. HyStem-C is fully chemically-defined and based on three biocompatible components: thiol-modified hyaluronan (Glycosil), thiol-reactive crosslinker, PEGDA (Extralink), and thiol-modified denatured collagen (Gelin-S). Gelin-S provides basic cell-attachment sites for a wide variety of primary cells and cell lines and is therefore recommended as an ideal substrate for adherent cell types and for cell culture optimization. In some cases, HyStem-C hydrogels can be further enhanced by the addition of ECM proteins to match native signals.
Promocell GmbH PromoCell Supplement Mix / Melanocyte Growth Medium
PromoCell Melanocyte Growth Medium is a PMA (Phorbol Myristate Acetate)-containing Medium and has been developed for the in vitro cultivation of juvenile melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with some experimental approaches. In these cases, we recommend using the PMA-free Melanocyte Growth Medium M3.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for equine, porcine, murine, and rat melanocytes.While the supplements of the Melanocyte Growth Medium are serum-free, the Basal Medium is manufactured with 0.2% serum.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Phorbol Myristate Acetate | 10 ng / ml |
STEMCELL Technologies StemSpan™ Lymphoid Progenitor Expansion Supplement (10X), Size: 5 mL
StemSpan™ NK Cell Generation Kit has been developed to differentiate CD34+ cells isolated from cord blood (CB) and bone marrow (BM) to natural killer (NK) cells, without use of stromal cells. This kit typically promotes the expansion of thousands of CD56+ NK cells in cultures initiated with CD34+ human CB cells. StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) contains a combination of recombinant human cytokines and other additives formulated to selectively promote the expansion and differentiation of CD34+ cells isolated from human CB and BM samples to lymphoid progenitor cells when used in combination with StemSpan™ SFEM II medium, and on plates coated with StemSpan™ Lymphoid Differentiation Coating Material (100X). Subsequently, StemSpan™ NK Cell Differentiation Supplement (100X) enables differentiation of lymphoid progenitor cells to NK cells.
STEMCELL Technologies StemSpan™ T Cell Progenitor Maturation Supplement (10X), Size: 12.5 mL
Supplement for the maturation of T cell progenitors to T cells
Research Products International Corp Agar, High Gel Strength, Powder, 10 KG
Purified high gel strength agar with excellent properties for tissue culture and microbiological work.
Molecular Biology Grade
Sigma Aldrich Fine Chemicals Biosciences Insulin solution human, 11061-68-0, MFCD00131380, 5mL
Chemically defined, recombinant, expressed in Saccharomyces cerevisiae, sterile-filtered, BioXtra, suitable for cell culture
Zymo Research Corporation Expansion Broth (EB), 100 ml
Uniquely formulated Expansion Broth™ (EB™) that is used in conjunction with our Overexpression Broth™ (OB™) for consistent high level recombinant protein expression.